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Related Experiment Videos

Cytosolic epoxide hydrolase.

J Meijer1, J W DePierre

  • 1Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, Sweden.

Chemico-Biological Interactions
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

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Cytosolic epoxide hydrolase, an enzyme in mammals, detoxifies harmful epoxides. Its presence in the cytosol may be an artifact, with potential peroxisomal localization, and its full function remains under investigation.

Area of Science:

  • Biochemistry
  • Enzymology
  • Xenobiotic Metabolism

Background:

  • Epoxide hydrolase activity is found in mammalian liver cytosol, with rats exhibiting low levels.
  • Cytosolic epoxide hydrolase (cEH) selectively hydrolyzes trans epoxides, complementing microsomal epoxide hydrolase.
  • cEH has been purified from mouse, rabbit, and human livers, with ongoing characterization of the mouse enzyme's properties.

Purpose of the Study:

  • To investigate the localization and properties of cytosolic epoxide hydrolase.
  • To understand the induction patterns and potential functional significance of cEH.
  • To clarify the relationship between cEH and peroxisomal enzymes.

Main Methods:

  • Enzyme activity assays in liver fractions.
  • Purification of cytosolic epoxide hydrolase.

Related Experiment Videos

  • Characterization of physicochemical and enzymatic properties.
  • Analysis of enzyme induction by xenobiotics and peroxisome proliferators.

    • Main Results:

    • cEH activity is present in the high-speed supernatant of mammalian liver cytosol.
    • Unlike other xenobiotic metabolizing enzymes, cEH is not induced by common inducers but is by peroxisome proliferators.
    • Observations suggest cEH might be localized in peroxisomes, with its cytosolic recovery being an artifact.

    Conclusions:

    • The recovery of cEH in the cytosolic fraction is likely an artifact due to peroxisome fragmentation.
    • cEH plays a role in detoxifying xenobiotic epoxides and may be involved in cellular defense against oxidative stress.
    • Further research is needed to elucidate the complete functional significance of cytosolic epoxide hydrolase.