Related Experiment Video
Updated: Dec 12, 2025
Nasal Wipes for Influenza A Virus Detection and Isolation from Swine
Published on: December 4, 2015
A molecular survey and phylogenetic analysis of porcine circovirus type 3 using oral fluid, faeces and serum
Jan Plut1, Urska Jamnikar-Ciglenecki2, Irena Golinar-Oven3
1Clinic for Ruminants and Pigs, Clinic for Reproduction and Farm Animals, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia. jan.plut@vf.uni-lj.si.
Background:
Porcine circovirus type 3 is the most recently discovered porcine circovirus, and an emerging pathogen. In this study the status of its presence on some Slovenian farms is reported. The effectiveness of the vaccine against porcine circovirus type 2 was assessed against porcine circovirus type 3. Group samples of oral fluid, faeces and individual serum samples were taken from six different pig categories and tested for presence of viral DNA, using both real time and conventional PCR. Positive samples were subjected to direct Sanger sequencing. Nucleotide sequences were analyzed and compared to GenBank PCV3 sequences.
Results:
Positive samples were sent for genome sequencing, which confirmed the presence of virus in all different pig categories on five farms. A high to moderate correlation of strong statistical significance was found between individual serum samples, oral fluid and faeces. Slovenian PCV3 was found to be distributed in a way similar to that of other countries. Slovenian PCV3 nt sequences are highly related, sharing more than 99.5% nt identity. On one farm a commercially available vaccine against porcine circovirus type 2 was used on 3-week-old pigs. It did not affect the presence of porcine circovirus type 3 in oral fluid or sera of any of the seven age groups of pigs, each with two control groups.
Conclusions:
The results constitute the first discovery of the virus in Slovenia. Genome sequencing has revealed a high degree of similarity between Slovenian and GenBank isolates.
