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Updated: Dec 12, 2025
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
An improved structure-switch aptamer-based fluorescent Pb2+ biosensor utilizing the binding induced quenching of AMT
Fenghua Geng1, Dandan Wang, Li Feng
1National Engineering Research Center of Coal Preparation and Purification, Key Laboratory of Coal Processing and Efficient Utilization of Ministry of Education, School of Chemical Engineering and Technology, China University of Mining & Technology, Xuzhou, 221116, China. 8422127@alu.fudan.edu.cn.
Abstract:
An improved aptamer-based fluorescent Pb2+ biosensor utilizing the binding induced quenching of AMT to G-quadruplex has been rationally designed with a LOD of 3.6 nM. The utility of the developed biosensor was demonstrated by the successful detection of Pb2+ in real complex clinical samples with satisfactory recovery and good reproducibility.
