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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.2K

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Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
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Subnanometer-scale imaging of nanobio-interfaces by frequency modulation atomic force microscopy.

Takeshi Fukuma1

  • 1Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.

Biochemical Society Transactions
|August 12, 2020
PubMed
Summary

Dynamic-mode atomic force microscopy (AFM) advances enable subnanometer visualization of biomolecules. Frequency modulation AFM (FM-AFM) and 3D-AFM reveal molecular structures and hydration, crucial for understanding nano-bio interfaces.

Keywords:
atomic force microscopyhydration structuressubnanoscale imaging

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Area of Science:

  • Biophysics
  • Nanotechnology
  • Biochemistry

Background:

  • Atomic Force Microscopy (AFM) has advanced significantly for biological studies.
  • Dynamic-mode AFM techniques offer high-resolution imaging of biomolecular structures.

Purpose of the Study:

  • To provide an overview of recent advancements in dynamic-mode AFM techniques.
  • To highlight the biological applications of these cutting-edge AFM methods.

Main Methods:

  • Frequency Modulation AFM (FM-AFM) for visualizing subnanometer surface structures of biomolecules.
  • Three-dimensional AFM (3D-AFM) combining high-resolution AFM with 3D tip scanning.
  • Visualization of 3D hydration structures and flexible surface structures at the nano-bio interface.

Main Results:

  • Direct visualization of protein secondary structures, DNA phosphate groups, and lipid-ion complexes using FM-AFM.
  • Subnanometer-scale resolution of 3D water distributions (hydration structures) on lipids, proteins, and DNAs via 3D-AFM.
  • Visualization of subnanometer 3D distributions of thermally fluctuating lipid headgroups using 3D-AFM.

Conclusions:

  • Dynamic-mode AFM techniques provide critical local information at nano-bio interfaces.
  • These advancements are vital for developing atomic- or molecular-scale models of interfacial structures and functions.
  • The presented techniques offer powerful tools for exploring biological systems at the nanoscale.