Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lipid polarity and sorting in epithelial cells.

G van Meer1, K Simons

  • 1European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.

Journal of Cellular Biochemistry
|January 1, 1988
PubMed
Summary

Epithelial cells maintain distinct apical and basolateral lipid compositions. A new model suggests lipids segregate into microdomains and bud into vesicles for targeted delivery to plasma membrane domains.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Feasibility, acceptability, and perceived benefits of a creative arts intervention for elementary school children living with speech, language and communication disorders.

Frontiers in child and adolescent psychiatry·2025
Same author

A feasibility study to investigate post-operative oxygen consumption (POpOC) after colorectal surgery requiring bowel resection.

Pilot and feasibility studies·2019
Same author

Circulating tumor DNA as a potential marker of adjuvant chemotherapy benefit following surgery for localized pancreatic cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2019
Same author

Short-term health effects in the general population following a major train accident with acrylonitrile in Belgium.

Environmental research·2016
Same author

Physiologic Changes in the Heart Following Cessation of Mechanical Ventilation in a Porcine Model of Donation After Circulatory Death: Implications for Cardiac Transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

A Novel von Hippel Lindau Gene Intronic Variant and Its Reclassification from VUS to Pathogenic: the Impact on a Large Family.

Journal of genetic counseling·2015

Area of Science:

  • Cell biology
  • Membrane biology
  • Epithelial biology

Background:

  • Epithelial cells have distinct apical and basolateral membrane domains.
  • The tight junction acts as a barrier preventing lipid intermixing.
  • The exact mechanism of lipid sorting remains unclear.

Purpose of the Study:

  • To propose a model for lipid sorting in epithelial cells.
  • To explain how unique lipid compositions are generated in apical and basolateral domains.
  • To investigate lipid sorting in Madin-Darby canine kidney (MDCK) cells.

Main Methods:

  • Utilized Madin-Darby canine kidney (MDCK) cells grown on filter supports.
  • Employed fluorescent lipid precursors to track lipid movement.
  • Analyzed lipid sorting within an intracellular compartment.

Main Results:

  • Apical and basolateral lipids are sorted intracellularly during biosynthesis.
  • Lipids segregate into distinct lateral microdomains.
  • These microdomains bud to form vesicles for domain-specific delivery.

Conclusions:

  • A novel model for lipid sorting in epithelial cells is presented.
  • Lateral segregation into microdomains is key to generating distinct membrane compositions.
  • Vesicle-mediated transport delivers lipids to specific plasma membrane domains.

Related Experiment Videos