Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Oogenesis

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Sex-specific metabolic and oxidative stress profiles associated to myocardial infarct size in mice after exposure to intermittent hypoxia.

Free radical biology & medicine·2025

Same author

Can a virtual reality tool detect minor executive functions impairment in patients with multiple sclerosis?

Revue neurologique·2025

Same author

Psychometric properties of a French translation of the Functionality Appreciation Scale in French adults (FAS).

Body image·2024

Same author

Neurological disorders related to recreational nitrous oxide abuse: Two sides of the same coin.

Revue neurologique·2023

Same author

Further characterization of clinical and laboratory features in VEXAS syndrome: large-scale analysis of a multicentre case series of 116 French patients.

The British journal of dermatology·2021

Same author

Effect of hydroxyurea exposure before puberty on sperm parameters in males with sickle cell disease.

Blood·2020

Search research articles

Related Experiment Video

Updated: Jun 9, 2026

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Published on: June 30, 2017

Regional adipocyte precursors in the female rat. Influence of ovarian factors.

G R Krakower¹, R G James, C Arnaud

¹Department of Medicine, Medical College of Wisconsin, Milwaukee 53226.

The Journal of Clinical Investigation

|March 1, 1988

Summary

Puberty increases differentiated preadipocytes and fat cell number in female rats. Ovarian hormones influence this fat cell growth and distribution, particularly in the femoral region.

More Related Videos

Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

Published on: August 16, 2020

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

Related Experiment Videos

Last Updated: Jun 9, 2026

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Published on: June 30, 2017

Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

Published on: August 16, 2020

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

Area of Science:

Endocrinology
Adipose Biology
Cell Biology

Background:

Adipose tissue expansion involves the differentiation of preadipocytes into adipocytes.
Understanding the regulation of preadipocyte differentiation is crucial for comprehending adipose tissue development and body fat distribution.

Purpose of the Study:

To develop a flow cytometric method to quantify differentiated and undifferentiated preadipocytes in rat adipose tissue.
To investigate the influence of puberty and ovarian hormones on regional preadipocyte populations and adipose tissue growth.

Main Methods:

Developed a flow cytometric immunofluorescence assay using an antibody specific to rat lipoprotein lipase (LPL).
Quantified differentiated (LPL-synthesizing) and undifferentiated preadipocytes in vascular-stromal fractions of various adipose depots.
Compared preadipocyte populations and depot sizes in pubertal vs. prepubertal female rats and ovariectomized rats.

Main Results:

Pubescence increased differentiated preadipocytes and fat cell number in perirenal, parametrial, dorsal, and femoral depots.
Undifferentiated preadipocyte percentage decreased with puberty in most depots, except the femoral.
Ovariectomy blunted pubertal adipose growth in femoral and parametrial depots and impaired the preservation of femoral undifferentiated preadipocytes.

Conclusions:

Ovarian factors significantly influence pubertal preadipocyte differentiation and adipocyte conversion in a region-specific manner.
The femoral adipose depot appears to possess an ovarian-dependent, self-renewing pool of fat cell precursors.
These findings suggest a mechanism linking ovarian hormones to body fat topography during sexual maturation.