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Updated: Dec 12, 2025
Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease
Published on: April 19, 2021
Novel D-π-A type near-infrared fluorescent probes for the detection of Aβ40 aggregates
Guanglei Lv1, Yunze Xu2, Jiajia Yang2
1Department of Neurology, Shanghai Zhoupu Hospital, Shanghai University of Medicine & Health Sciences, Shanghai, 201318, China. sunay@sumhs.edu.cn and Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, Zhejiang Normal University, Jinhua 321004, Zhejiang, P. R. China. cxli@sdu.edu.cn.
Abstract:
Aberrant accumulation of Amyloid-β (Aβ) peptide is closely related to Alzheimer's disease. Thus, it is important to develop featured probes for the specific detection of Aβ species. Herein, we designed and synthesized a novel near-infrared fluorescent probe SDPY based on the D-π-A architecture for the detection of Aβ aggregates. The probe SDPY displayed higher affinity for Aβ40 aggregates over Aβ42 aggregates in solution (Kd = 164 nM vs. 2.1 μM). In addition, SDPY showed excellent anti-interference against a wide range of other substances. Furthermore, SDPY was capable of labeling Aβ40 aggregates better than Aβ42 aggregates in the brain sections of AD transgenic mouse models.
