Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.5K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Role of Polymer Encapsulation in Optimizing Donor-Acceptor Organic Nanoparticles for Efficient Cancer Phototherapy.

International journal of molecular sciences·2026
Same author

From Small Molecules to Polymers: Developing Non-Fullerene Acceptors for Efficient NIR Photothermal Cancer Therapy.

Polymers·2025
Same author

Photoactivatable Cisplatin-Combretastatin-BODIPY Pt(IV) Prodrug for Dual-Action Light-Controlled Chemotherapy.

ACS pharmacology & translational science·2025
Same author

Metal/metal oxide nanoparticles with antibacterial activity and their potential to disrupt bacterial biofilms: Recent advances with emphasis on the underlying mechanisms.

Advances in colloid and interface science·2025
Same author

Game of ligands: tuning the photodynamic activity of P(V) porphyrins in aqueous media.

Dalton transactions (Cambridge, England : 2003)·2025
Same author

Photocrosslinking of hyaluronic acid-based hydrogels through biotissue barriers.

Biomaterials science·2025

Related Experiment Video

Updated: Dec 12, 2025

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
13:51

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

15.7K

A versatile platform for bioimaging based on colominic acid-decorated upconversion nanoparticles.

Polina A Demina1, Natalya V Sholina, Roman A Akasov

  • 1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Moscow, 117997 Russia. a-generalova@yandex.ru.

Biomaterials Science
|August 12, 2020
PubMed
Summary

Colominic acid coating enhances lanthanide-doped upconversion nanoparticles (UCNPs) for bioimaging. This modification improves circulation time and accumulation at inflammation sites, enabling effective in vivo imaging.

More Related Videos

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

10.2K
An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
11:20

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

7.7K

Related Experiment Videos

Last Updated: Dec 12, 2025

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
13:51

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

15.7K
A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

10.2K
An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
11:20

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

7.7K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Materials Science

Background:

  • Lanthanide-doped upconversion nanoparticles (UCNPs) are advanced bioimaging agents due to their deep tissue penetration and high signal-to-noise ratio under near-infrared excitation.
  • Effective surface functionalization is crucial for the in vivo application of UCNPs, impacting their biocompatibility and circulation dynamics.

Purpose of the Study:

  • To develop a novel surface modification strategy for UCNPs using colominic acid to impart stealth properties for enhanced bioimaging.
  • To evaluate the biocompatibility, protein adsorption, cellular uptake, and in vivo performance of colominic acid-functionalized UCNPs.

Main Methods:

  • A two-step surface functionalization strategy involving hydrophilization with polyethyleneimine followed by colominic acid attachment (electrostatic or covalent).
  • In vitro assessment of cytotoxicity, non-specific blood protein adsorption, and macrophage uptake.
  • In vivo evaluation of circulation time, biodistribution, and inflammation imaging in small animal models.

Main Results:

  • Colominic acid coating rendered UCNPs non-cytotoxic with minimal non-specific protein adsorption and low macrophage uptake.
  • Colominic acid-functionalized UCNPs exhibited significantly prolonged circulation times (up to 3 hours) compared to polyethylene glycol (PEG) coatings.
  • Efficient accumulation of UCNPs at inflammation sites was observed, facilitating whole-body inflammation imaging and in vivo blood vessel visualization.

Conclusions:

  • Colominic acid is a highly effective, non-toxic coating for UCNPs, conferring desirable stealth properties for bioimaging applications.
  • The enhanced circulation and targeted accumulation of colominic acid-UCNPs improve their utility for in vivo imaging of inflammatory processes.
  • This study validates colominic acid as a superior surface modifier for UCNPs, advancing their potential in biomedical diagnostics.