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Long non-coding RNA uc.80- overexpression promotes M2 polarization of microglias to ameliorate depression in rats

Xun-Hu Gu1, Li-Jun Xu1, Li-Li Zheng2

  • 1Department of Neurology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.

IUBMB Life
|August 12, 2020
PubMed

Insights

Long non-coding RNA uc.80- overexpression alleviates depression in rats by promoting beneficial M2 microglia polarization. This suggests uc.80- as a potential therapeutic target for depression treatment.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Microglia polarization plays a role in depression pathogenesis.
  • Long non-coding RNA uc.80- is downregulated in the hippocampus of depressed rats.

Purpose of the Study:

  • To investigate the role of uc.80- in microglia polarization in depression.
  • To explore uc.80- as a potential therapeutic target for depression.

Main Methods:

  • Established a rat model of depression using chronic unpredictable mild stress (CUMS).
  • Analyzed microglia polarization (M1/M2) and uc.80- expression in rat hippocampi.
  • Assessed behavioral changes and hippocampal neuron apoptosis in response to uc.80- overexpression in vivo and in vitro.

Main Results:

  • Depressed rats showed increased M1 and decreased M2 microglia in the hippocampus.
  • uc.80- was significantly downregulated in depressed rat hippocampi.
  • uc.80- overexpression alleviated depressive behaviors, promoted M2 microglia polarization, and reduced hippocampal neuron apoptosis.

Conclusions:

  • lncRNA uc.80- overexpression ameliorates depression in rats.
  • This effect is mediated by promoting M2 microglia polarization.
  • uc.80- represents a potential therapeutic target for depression.
Keywords:
CUMSdepressionmicrogliapolarizationuc.80-

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