Influence of temperature on COD_Mn and Mn²⁺ removal and microbial community structure in pilot-scale biofilter

Test water temperature (TWT) is a significant operational parameter in biofilter. In this study, a pilot-scale biofilter was established to investigate the removal efficiency of COD_Mn and Mn²⁺ and the microbial community structure at different TWT. When COD_Mn and Mn²⁺ in the influent were 6-8 and 0.9-1.2 mg/L, respectively, the removal rates were 22.61% and 94.28% at the low TWT, while 69.42% and 97.85% at the high TWT, respectively. Biological COD_Mn and Mn²⁺ removal followed the first-order reaction, and at the low and high TWT, the k value was 0.00704 and 0.0738 and 0.0313 and 0.113 min^-1, respectively. Organic matter oxidizing bacteria (OMOB, Sphingopyxis, Sphingomonas, Amphiplicatus, Novosphingobium, Gemmatimonas, Chryseolinea and Sphingobium) and manganese oxidizing bacteria (MnOB, Hyphomicrobium, Pedomicrobium and Pseudomonas) were coexisted in 0-1.5 m of the biofilter bed at the low and high TWT, and the abundances were not the main factor affecting the removal efficiency, however the activity.