Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Proteasome

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Biodegradable Acoustic Targeting for Ultrasound-Supported Gene Therapy (BATUS) in Glioblastoma.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Wireless knee joint monitoring using biodegradable single-ended pressure sensor in osteoarthritis management.

Science advances·2026

Same author

AI-assisted vocal emotion analysis in forensic interview with children: an exploratory study.

Frontiers in psychology·2026

Same author

Bioprinted human skin equivalent for evaluating non-invasive monopolar radiofrequency treatment: a novel approach.

Lasers in medical science·2026

Same author

Anti-inflammatory effects of taurocholic acid and tauroursodeoxycholic acid from rainbow trout spleen extract via NF-κB suppression.

Scientific reports·2026

Same author

Deep Learning-Based Acoustic Screening for Penetration-Aspiration Events Using Short Voice Recordings.

Dysphagia·2026

Search research articles

Related Experiment Video

Updated: Dec 12, 2025

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

Assay Systems for Profiling Deubiquitinating Activity.

Jinhong Cho¹, Jinyoung Park², Eunice EunKyeong Kim¹

¹Biomedical Research Institute, Korea Institute of Science and Technology, Seoul 02792, Korea.

International Journal of Molecular Sciences

|August 13, 2020

Summary

Methods to assess deubiquitinating enzyme (DUB) activity are crucial for identifying novel DUBs and understanding their roles in disease. This review covers in vitro and in vivo techniques for evaluating DUB activity and developing targeted inhibitors.

Keywords:

Ub-probes activity-based probes cell permeability deubiquitination assay

More Related Videos

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay

Published on: September 27, 2024

Related Experiment Videos

Last Updated: Dec 12, 2025

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay

Published on: September 27, 2024

Area of Science:

Biochemistry
Molecular Biology
Enzymology

Background:

Deubiquitinating enzymes (DUBs) are critical regulators of cellular processes, including protein degradation and localization.
Dysregulated DUB activity is implicated in various diseases, yet many DUB functions remain uncharacterized.
Assessing DUB activity is vital for discovering new DUBs and understanding their roles.

Purpose of the Study:

To review existing and novel methods for evaluating deubiquitinating enzyme (DUB) activity.
To discuss various probes used for DUB activity assessment in vitro and in vivo.
To highlight techniques for visualizing and quantifying DUB activity in live cells.

Main Methods:

Review of established biochemical assays for DUB activity using cell lysates or purified enzymes.
Discussion of different probe types, including activity-based probes (ABPs).
Introduction of cell-based assays and cell-permeable ABPs for live-cell imaging and quantification.

Main Results:

Comprehensive overview of diverse methodologies for DUB activity evaluation.
Categorization of probes based on their application in vitro and in vivo.
Demonstration of techniques enabling direct visualization and quantification of DUB activity in live cells.

Conclusions:

Effective assessment of DUB activity is essential for disease research and drug development.
The reviewed methods and probes provide valuable tools for characterizing DUBs.
This review facilitates the development of novel DUB inhibitors by informing probe selection and application.