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Related Experiment Video

Updated: Dec 12, 2025

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Published on: March 31, 2021

Cranberry extract-based formulations for preventing bacterial biofilms.

Ashlee C Greene¹, Abhinav P Acharya^1,2, Sang B Lee¹

¹Department of Chemical Engineering, University of Pittsburgh, Pittsburgh, PA, 15261, USA.

Drug Delivery and Translational Research

|August 13, 2020

Summary

Developing effective delivery systems for natural compounds like cranberry extract is difficult. New formulations using stearic acid with PVP or LAE offer high loading and sustained release, successfully inhibiting biofilm formation.

Keywords:

Biofilms Controlled release systems Cranberry extract Formulation Oral drug delivery

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Last Updated: Dec 12, 2025

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Area of Science:

Biomaterials Science
Natural Product Chemistry
Oral Health Research

Background:

Formulating complex natural extracts, such as cranberry extract, presents challenges due to unknown active components and potential interactions.
Cranberry extracts are investigated for preventing biofilm formation on dental surfaces, but their diverse composition complicates targeted delivery.

Purpose of the Study:

To develop novel formulations for high-loading, sustained release of unrefined cranberry extract for biofilm inhibition.
To compare the efficacy of poly(lactic-co-glycolic) acid (PLGA) microencapsulation with stearic acid-based formulations.

Main Methods:

Candidate formulations were generated using cranberry extract (CE15) with PLGA microencapsulation.
Alternative formulations involved complexing CE15 with stearic acid and either polyvinylpyrrolidone (PVP) or Ethyl lauroyl arginate (LAE).
Formulation loading capacity, release kinetics, and biofilm inhibition efficacy were evaluated.

Main Results:

Stearic acid-based formulations (with PVP or LAE) achieved higher loading of CE15 components compared to PLGA microencapsulation.
These formulations demonstrated tunable, sustained release of the cranberry extract.
Two successful formulations, one preventative and one treatment-based, effectively inhibited biofilm growth in vitro.

Conclusions:

Stearic acid in combination with PVP or LAE provides an effective method for high-loading, sustained-release delivery of complex natural extracts like cranberry.
This formulation strategy shows promise for developing advanced oral care products and other natural extract delivery systems.