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Strain-specific differences in behaviour among Lacticaseibacillus rhamnosus cell wall mutants during direct

Eline Byl1, Katarina Jokicevic1, Shari Kiekens1

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Probiotic cell surface molecules, like exopolysaccharides, protect Lacticaseibacillus rhamnosus GG during tablet compression. This finding is crucial for developing effective oral probiotic formulations with viable cells.

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3-D modelCompressionLactobacillus caseiLactobacillus rhamnosusProbioticsTabletTableting propertiesViability

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Area of Science:

  • Microbiology
  • Biotechnology
  • Pharmaceutical Sciences

Background:

  • The human microbiome plays a critical role in health, and imbalances are linked to various disorders.
  • Probiotics, particularly bacteria, are used to restore microbial balance and modulate immune functions.
  • Tablets are a preferred oral dosage form for probiotics, but cell viability during manufacturing is a challenge.

Purpose of the Study:

  • To investigate the role of cell surface molecules in the survival of Lacticaseibacillus rhamnosus GG during direct compression tablet manufacturing.
  • To understand how exopolysaccharides and lipoteichoic acids influence probiotic cell survival under tableting stress.

Main Methods:

  • Spray-dried powders of L. rhamnosus GG, an exopolysaccharide-deficient mutant, and a lipoteichoic acid mutant were used.
  • These strains were blended with different filler-binders and compacted under varying compression pressures.
  • Cell survival rates and tableting properties were analyzed under each condition.

Main Results:

  • Exopolysaccharides on L. rhamnosus GG act as a protective shield, significantly enhancing survival during direct compression.
  • D-alanylation of lipoteichoic acids also plays a protective role, though its absence had less impact on survival related to tableting properties.
  • The sensitivity of probiotic strains to tableting conditions is species and strain-dependent.

Conclusions:

  • Cell surface molecules are critical for the survival of L. rhamnosus GG during probiotic tablet production.
  • Exopolysaccharides are key protective components, essential for maintaining probiotic viability in solid dosage forms.
  • Understanding strain-specific surface properties is vital for optimizing probiotic tablet manufacturing.