Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

969
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
969
Controlled-Current Coulometry: Coulometric Titration01:18

Controlled-Current Coulometry: Coulometric Titration

409
Coulometric titrations are a form of titrimetric analysis where the reagent is generated electrically, and its amount is evaluated based on current and generating time. The electron serves as the standard reagent. The procedure is similar to conventional titrations, such as endpoint detection.
The fundamental requirements for coulometric titrations are (1) 100% efficiency in the reagent-generating electrode reaction and (2) a stoichiometric and preferably rapid reaction between the generated...
409
Controlled-Current Coulometry: Overview01:27

Controlled-Current Coulometry: Overview

532
Controlled current coulometry, also known as amperostatic coulometry, is a technique used in electrochemical analysis to measure the quantity of a substance through the controlled passage of current. It involves the application of a constant current to an electrochemical cell containing the analyte of interest. As the current flows through the cell, the analyte undergoes a redox reaction at the electrode surface, resulting in a charge transfer. By monitoring the time required for a certain...
532
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

690
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
690
Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

528
Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential...
528

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Icing in the Cake: Water in Nanoscopic Confinement by Cellulose.

The journal of physical chemistry. B·2025
Same author

Decoupling rheology from particle concentration by charge modulation: Aqueous graphene-clay dispersions.

Journal of colloid and interface science·2023
Same author

Fast Depolymerization of PET Bottle Mediated by Microwave Pre-Treatment and An Engineered PETase.

ChemSusChem·2023
Same author

Fast Depolymerization of PET Bottle Mediated by Microwave Pre-Treatment and An Engineered PETase.

ChemSusChem·2023
Same author

Water as an Intrinsic Structural Element in Cellulose Fibril Aggregates.

The journal of physical chemistry letters·2022
Same author

Timescales for convergence in all-atom molecular dynamics simulations of hydrated amorphous xylan.

Carbohydrate polymers·2022

Related Experiment Video

Updated: Dec 12, 2025

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

6.0K

Improved accuracy and precision in electrophoretic NMR experiments. Current control and sample cell design.

Yuan Fang1, Pavel V Yushmanov2, István Furó1

  • 1Division of Applied Physical Chemistry, Department of Chemistry, KTH Royal Institute of Technology, SE-10044 Stockholm, Sweden.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|August 14, 2020
PubMed
Summary

Electrophoretic NMR (ENMR) can yield unique data but suffers from artifacts. Simple modifications, including current-controlled pulses and porous plugs, significantly enhance ENMR accuracy and precision.

Keywords:
Effective chargeElectrophoretic NMRElectrophoretic mobilityIon mobility

More Related Videos

Precise Electrochemical Sizing of Individual Electro-Inactive Particles
05:03

Precise Electrochemical Sizing of Individual Electro-Inactive Particles

Published on: August 4, 2023

1.6K
Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
10:25

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

Published on: March 9, 2021

3.6K

Related Experiment Videos

Last Updated: Dec 12, 2025

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

6.0K
Precise Electrochemical Sizing of Individual Electro-Inactive Particles
05:03

Precise Electrochemical Sizing of Individual Electro-Inactive Particles

Published on: August 4, 2023

1.6K
Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
10:25

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

Published on: March 9, 2021

3.6K

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Spectroscopy

Background:

  • Electrophoretic NMR (ENMR) offers unique insights into physico-chemical properties.
  • Experimental artifacts like thermal convection and electrolytic byproducts limit ENMR accuracy.
  • Existing ENMR methods often require calibration with known standards.

Purpose of the Study:

  • To present simple hardware and protocol modifications for ENMR.
  • To significantly improve the accuracy and precision of ENMR measurements.
  • To reduce common experimental artifacts in ENMR.

Main Methods:

  • Utilizing a symmetric sample cell with controlled current feeding.
  • Implementing a porous plug within the sample cell.
  • Employing current-controlled pulses instead of voltage-controlled pulses for electric field application.

Main Results:

  • Demonstrated significant reduction in thermal convection and electrolytic artifacts.
  • Achieved enhanced experimental accuracy and precision in ENMR measurements.
  • Validated the effectiveness of current-controlled pulses in artifact mitigation.

Conclusions:

  • Modified ENMR protocols with current-controlled pulses and porous plugs substantially improve data quality.
  • These modifications offer a more robust and reliable method for physico-chemical analysis using ENMR.
  • The approach eliminates the need for calibration with samples of known electrophoretic mobility.