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Localization of ZIP14 and ZIP8 in HIBCPP Cells.

Shannon E Morgan1, Horst Schroten2, Hiroshi Ishikawa3

  • 1Department of Nutritional Sciences, University of Arizona, Tucson, AZ 85721, USA.

Brain Sciences
|August 14, 2020
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Summary

Researchers identified manganese transporters ZIP14 and ZIP8 in a human choroid plexus model. This finding advances understanding of manganese homeostasis and transport across the blood-cerebrospinal fluid barrier.

Keywords:
Blood-cerebrospinal fluid barrierHIBCPPManganeseSLC39A14SLC39A8ZIP14ZIP8

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Physiology

Background:

  • The blood-cerebrospinal fluid barrier (BCB) is crucial for maintaining brain manganese (Mn) homeostasis.
  • This barrier comprises polarized epithelial cells with tight junctions, regulating nutrient passage via transporters.

Purpose of the Study:

  • To establish a polarized model of the human choroid plexus epithelial barrier.
  • To identify and localize manganese importers in this model.

Main Methods:

  • Established a polarized human choroid plexus papilloma cell line (HIBCPP) model.
  • Investigated the presence and localization of manganese importers ZIP14 and ZIP8 within the HIBCPP cell monolayer.

Main Results:

  • Successfully established a polarized HIBCPP cell model.
  • Identified ZIP14 and ZIP8 as manganese importers in HIBCPP cells.
  • Found ZIP14 enriched at the basolateral side and ZIP8 at the apical side of the cell monolayer.

Conclusions:

  • The study provides the first identification and localization of Mn importers ZIP14 and ZIP8 in a human BCB model.
  • This research enhances understanding of Mn transport mechanisms across the BCB.
  • Findings contribute to elucidating the regulation of Mn homeostasis in the brain.