Related Experiment Video

Updated: Dec 12, 2025

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
10:37

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

8.6K

Differential miRNA-Gene Expression in M Cells in Response to Crohn's Disease-Associated AIEC

Anaïs Larabi1, Laurène Salesse1, Charlotte Cordonnier1

  • 1M2iSH, UMR 1071 Inserm, Université Clermont Auvergne, INRAE USC 2018, CRNH, 63001 Clermont-Ferrand, France.

Microorganisms
|August 14, 2020
PubMed

Insights

Adherent-invasive Escherichia coli (AIEC) alters gene and microRNA (miRNA) expression in intestinal cells. This study reveals miRNA-mediated gene regulation during AIEC infection, enhancing understanding of Crohn's disease pathogenesis.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Molecular Biology

Background:

  • Adherent-invasive Escherichia coli (AIEC) are implicated in Crohn's disease (CD) pathogenesis.
  • AIEC colonize the ileal mucosa and can invade intestinal epithelial cells (IECs) and M cells.
  • Gene and microRNA (miRNA) expression changes in response to AIEC infection in IECs and M cells are largely uncharacterized.

Purpose of the Study:

  • To comprehensively analyze miRNA and gene expression profiles in human intestinal epithelial Caco-2 cells and an in vitro M-cell model.
  • To investigate the impact of AIEC LF82 strain infection on these profiles under basal and infected conditions.
  • To identify potential miRNA-mediated gene regulation mechanisms during AIEC infection.

Main Methods:

  • Utilized human intestinal epithelial Caco-2 monolayers and an in vitro M-cell model.
  • Performed comprehensive miRNA and gene profiling under basal and AIEC LF82 infected conditions.
  • Conducted in silico analysis to predict miRNA-gene interactions.

Main Results:

  • AIEC LF82 translocated through M cells but not Caco-2 monolayers.
  • Identified differential gene expression and miRNA profiles between M cells and Caco-2 cells.
  • Observed AIEC infection-induced changes in gene and miRNA profiles in both cell types.
  • Discovered potential miRNA-mediated regulation of AIEC-dysregulated genes.

Conclusions:

  • AIEC infection significantly alters gene and miRNA expression in intestinal epithelial and M cells.
  • A miRNA-mediated regulatory mechanism is suggested in response to AIEC infection.
  • This study provides insights into M cell biology and AIEC-host interactions relevant to Crohn's disease.
Keywords:
Adherent-invasive E. coliCrohn diseaseM cellsMicroRNAsTranscriptomic analysis

More Related Videos

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

6.4K
Evaluating Therapeutic Interventions in the SHIP-deficient Mouse Model of Crohn Disease-like Ileitis and Fibrosis09:44

Evaluating Therapeutic Interventions in the SHIP-deficient Mouse Model of Crohn Disease-like Ileitis and Fibrosis

Published on: October 14, 2025

232

Related Concept Videos

Inflammatory Bowel Disease II: Crohn's Disease01:30

Inflammatory Bowel Disease II: Crohn's Disease

Introduction
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
784
Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF01:24

Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF

Tumor Necrosis Factor (TNF), a proinflammatory cytokine, contributes significantly to the inflammation seen in Crohn's disease. It exists as soluble TNF and membrane-bound TNF, with actions mediated through TNF receptors (TNFR). TNFR activation leads to the release of proinflammatory cytokines, T-cell activation, collagen production, and leukocyte migration, all contributing to inflammation in Crohn's disease. Anti-TNF monoclonal antibodies, namely infliximab (Remicade), adalimumab...
350
Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents01:29

Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents

Crohn's disease is an inflammatory bowel disorder marked by chronic inflammation of the GI tract. Various treatment strategies for Crohn's disease are employed, such as immunomodulatory agents, glucocorticoids, and biologics or anti-TNF therapy. Azathioprine (Imuran), a commonly used immunomodulatory drug for Crohn's disease, is converted in the body to mercaptopurine, which inhibits purine biosynthesis and cell proliferation. Both are utilized in severe cases of Inflammatory Bowel...
360