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Small Non-Coding RNAome of Ageing Chondrocytes
Panagiotis Balaskas1, Jonathan A Green2, Tariq M Haqqi2
1Institute of Life Course and Medical Sciences, William Henry Duncan Building, 6 West Derby Street, Liverpool L7 8TX, UK.
International Journal of Molecular Sciences
|August 14, 2020
Summary
Ageing significantly alters small non-coding RNA (sncRNA) expression in equine cartilage. Specific tRNA fragments, crucial for cartilage health, decrease with age and in osteoarthritis.
Area of Science:
- Molecular Biology
- Genomics
- Biochemistry
Background:
- Ageing is a primary risk factor for osteoarthritis.
- Limited research exists on ageing's impact on small non-coding RNA (sncRNA) expression in cartilage.
Purpose of the Study:
- To investigate the effect of ageing on sncRNA expression in equine chondrocytes.
- To explore the potential role of sncRNAs in age-related cartilage diseases.
Main Methods:
- Small RNA sequencing (RNA-seq) of young and old equine chondrocytes.
- Differential expression analysis using DESeq2.
- Validation of selected sncRNAs (miRNAs, snoRNAs) via qRT-PCR.
- Analysis of tRNA fragments in equine and human osteoarthritis cartilage.
Main Results:
- 83 differentially expressed sncRNAs were identified between young and old equine chondrocytes.
- Expression of specific tRNA halves (tiRNAs), tiRNA-5035-GluCTC and tiRNA-5031-GluCTC-1, was reduced in aged equine chondrocytes and human osteoarthritis cartilage.
- This study provides the first comprehensive analysis of ageing effects on sncRNA expression in equine chondrocytes.
Conclusions:
- Ageing induces significant changes in various sncRNA species within cartilage.
- Altered sncRNA expression, particularly tRNA fragments, may contribute to age-related cartilage degeneration and osteoarthritis.
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