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Updated: Dec 12, 2025

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
Published on: January 31, 2025
Multiomic analysis of a dried single-drop plasma sample using an integrated mass spectrometry approach
Weina Gao1, Qiaoyun Zhang, Yiran Su
1Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, China. tianrj@sustech.edu.cn.
Abstract:
An easy-to-use and fast approach was developed for integrated proteomic and metabolic profiling in a dried single-drop plasma sample. Plasma collection, room temperature storage, and sample preparation for both proteins and metabolites were seamlessly integrated in one spintip device. MS-based multiomic profiling using the same nano LC-MS system identified more than 150 proteins and 160 metabolites from the 1 μL plasma sample in 6 hours. Further combination with micro-flow LC and targeted MS made it a promising approach for the fast profiling of molecular biomarkers with high sensitivity and accuracy.
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