Molecular biohydrogen production by dark and photo fermentation from wastes containing starch: recent advancement and...

Changing lifestyle is increasing the energy demand. Fossil fuel is unable to deliver such huge energy. Clean energy from renewable source can solve this problem. Hydrogen is a clean and energy-efficient fuel and used for electricity generation by fuel cells or can be used in combustion engine. Easy availability of starch wastes from different industrial food processing wastes makes it a potential source for hydrogen (H₂) generation. Among various processes such as steam reforming, electrolysis, biophotolysis of water and anaerobic fermentation, anaerobic fermentation technique is environmentally friendly and requires less external energy, making it a preferred process for H₂ generation. Dark fermentation process can use wide range of substrates including agricultural and industrial starchy waste with low level of undesirable compounds. Application of both anaerobic dark and photofermentation can improve H₂ yield and production rate. H₂ production from wastes containing starch serves dual benefit of waste reduction and energy generation. As starch is a polymer and all hydrogen-producing bacteria cannot produce amylase to hydrolyze it, a pretreatment step is required to convert starch into glucose and maltose. In this present review paper, we have summarized: (i) potential of various types of starch-containing wastes as feedstock, (ii) various fermentation techniques, (iii) optimization of external process parameter, (iv) application of bioreactor and simulation in fermentation technique and (v) advancement in H₂ production from starchy wastes.