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Updated: Dec 12, 2025

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
Published on: July 20, 2016
Further Exploration of Hydrazine-Mediated Bioconjugation Chemistries
Melrose Mailig1, David Hymel1, Fa Liu1
1Novo Nordisk Research Center, 530 Fairview Avenue North, Seattle, Washington 98109, United States.
Abstract:
The hydrazine group serves as a great anchor for bioconjugation; however, the application of hydrazone ligation has been limited by poor product stability. We aim to resolve such issues by optimizing the recently established pyrazolone ligation and investigating a new pyrazole ligation. We have identified a new, electron-deficient pyrazolone ligation and a regiospecific pyrazole ligation, both offering aqueous buffer stable and chemically inert products possessing triazole-like structures while not involving any heavy metal catalyst.
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