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Aggregation Enhanced Responsiveness of Rationally Designed Probes to Hydrogen Sulfide for Targeted Cancer Imaging.

Rongchen Wang¹, Xianfeng Gu², Qizhao Li¹

¹Key Laboratory for Advanced Materials and Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.

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Researchers developed novel molecular probes that self-assemble into nanoprobes. These probes enhance near-infrared fluorescence and photoacoustic signals in response to hydrogen sulfide (H₂S), enabling targeted in vivo cancer imaging.

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Area of Science:

Biomedical Engineering
Molecular Imaging
Nanotechnology

Background:

Activatable molecular probes are crucial for targeted cancer imaging.
Hydrophobic probes often aggregate in aqueous media, hindering their responsiveness and practical application.

Purpose of the Study:

To develop novel small molecular probes with enhanced responsiveness to hydrogen sulfide (H₂S) for in vivo imaging of H₂S-rich cancers.
To address the aggregation issue of conventional probes in aqueous environments.

Main Methods:

Designed two small molecular probes by modulating hydrophilicity-lipophilicity balance using N-methylpyridinium.
Investigated the spontaneous self-assembly into nanoprobes under physiological conditions.
Evaluated probe performance for near-infrared fluorescence and photoacoustic signal generation upon H₂S activation.

Main Results:

The designed probes spontaneously self-assemble into nanoprobes in physiological conditions.
Aggregated probes exhibit enhanced near-infrared fluorescence and photoacoustic signals upon specific H₂S activation.
Enabled in vivo visualization and differentiation of cancers based on H₂S content.

Conclusions:

Developed a novel design strategy for activatable molecular probes with aggregation-enhanced responsiveness.
Demonstrated the potential for in vivo imaging and precision cancer diagnostics using H₂S-responsive nanoprobes.
The strategy paves the way for optimized probe design for improved cancer detection.