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Ultrabright and Serum-Stable Squaraine Dyes.
Yogesh Yadav1, Eric Owens1,2, Shinsuke Nomura3,4
1Department of Chemistry, Georgia State University, 145 Piedmont Avenue SE, Atlanta, Georgia 30303, United States.
Journal of Medicinal Chemistry
|August 14, 2020
Summary
New squaraine fluorophores with an internal salt bridge offer enhanced stability and optical properties. These novel compounds show promise as improved imaging agents and for biological tagging applications.
Area of Science:
- Organic Chemistry
- Materials Science
- Biophotonics
Background:
- Squaraine dyes are known for their optical properties but suffer from environmental instability.
- Developing stable fluorophores is crucial for advanced imaging and sensing applications.
Purpose of the Study:
- To synthesize highly stable symmetric and asymmetric squaraine fluorophores.
- To evaluate their improved optical characteristics and stability for potential applications.
Main Methods:
- Synthesis of novel squaraine fluorophores featuring an internal salt bridge.
- Characterization of molar absorptivity, quantum yield in serum, and thermal/photochemical stability.
- Assessment of covalent attachment for biological tagging.
Main Results:
- Successfully synthesized stable symmetric and asymmetric squaraine fluorophores.
- Observed increased molar absorptivity, quantum yield in serum, and stability compared to existing dyes.
- Demonstrated potential for biological tagging without compromising optical properties.
Conclusions:
- The newly designed squaraine fluorophores exhibit superior stability and optical performance.
- These compounds represent a significant advancement for squaraine dyes in imaging and sensing.
- The covalent attachment strategy enables versatile biological applications.
