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High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings
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Real-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric Micelles.

Rafael Piñol1, Justyna Zeler2,3, Carlos D S Brites2

  • 1ICMA, Institute of Materials Science of Aragon, CSIC, University of Zaragoza, 50008 Zaragoza, Spain.

Nano Letters
|August 14, 2020
PubMed
Summary

Researchers developed a new method for mapping intracellular temperature in breast cancer cells. This technique reveals significant temperature variations within cells, aiding the study of cellular thermogenesis.

Keywords:
Intracellular temperature mappingLanthanide-based polymer nanomicellesLuminescence thermometryThermogenesis

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Area of Science:

  • Cellular Biophysics
  • Nanotechnology
  • Cancer Research

Background:

  • Measuring intracellular temperature is challenging due to complex cellular environments and heat transfer.
  • Understanding cellular thermogenesis is crucial for studying various biological processes and diseases.

Purpose of the Study:

  • To develop a novel system for precise intracellular temperature mapping.
  • To investigate temperature heterogeneity within individual breast cancer cells.

Main Methods:

  • Utilized rationally designed luminescent lanthanide (Ln3+)-bearing polymeric micellar probes (Ln = Sm, Eu).
  • Employed a fluorescence microscope system with a temperature uncertainty of 0.2 K.
  • Incubated probes in MDA-MB468 breast cancer cells and recorded 2D thermal images.

Main Results:

  • Observed inhomogeneous intracellular temperature progressions up to ~20 degrees.
  • Detected subcellular temperature gradients of ~5 degrees between the nucleolus and other cellular regions.
  • Demonstrated significant temperature differences in response to external medium temperature changes (296–304 K).

Conclusions:

  • The developed luminescent probe system enables accurate intracellular temperature mapping.
  • Significant intracellular temperature gradients highlight the thermogenic activity of organelles.
  • This tool has potential for studying dynamic intracellular processes and cellular metabolism.