Related Experiment Video
Updated: Dec 12, 2025

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
Published on: February 10, 2022
Specific Buffer Effects on the Intermolecular Interactions among Protein Molecules at Physiological pH
Andrea Salis1,2,3, Luca Cappai1, Cristina Carucci1,2,3
1Department of Chemical and Geological Sciences, University of Cagliari, and Centro NanoBiotecnologie Sardegna (CNBS), Cittadella Universitaria, SS 554 bivio Sestu, 09042 Monserrato (CA), Italy.
Abstract:
BSA and lysozyme molecular motion at pH 7.15 is buffer-specific. Adsorption of buffer ions on protein surfaces modulates the protein surface charge and thus protein-protein interactions. Interactions were estimated by means of the interaction parameter kD obtained from plots of diffusion coefficients at different protein concentrations (Dapp = D0[1 + kDCprotein]) via dynamic light scattering and nuclear magnetic resonance. The obtained results agree with recent findings confirming doubts regarding the validity of the Henderson-Hasselbalch equation, which has traditionally provided a basis for understanding pH buffers of primary importance in solution chemistry, electrochemistry, and biochemistry.
More Related Videos
10:41Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
Published on: March 7, 2018
07:22How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
Published on: February 11, 2019
Related Concept Videos
Protein Buffers in Blood Plasma and Cells
Certain amino acids can exist in a zwitterion state at a...
Buffer Effectiveness
The buffer capacity is the amount of acid or base that can be added to a given volume...
Buffer Systems in the Body
A typical buffer system in bodily fluids includes a weak acid and its corresponding...
Factors Affecting Protein-Drug Binding: Drug Interactions
Displacement interactions can have varying outcomes, ranging from toxicity to virtually...
Physiological Pharmacokinetic Models: Assumption with Protein Binding
The Equilibrium Binding Constant and Binding Strength