Related Experiment Video
Updated: Dec 12, 2025
Translational Orthotopic Models of Glioblastoma Multiforme
Published on: February 17, 2023
Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model
Kristell Oizel1, Chendong Yang1, Ophelie Renoult2
1Children's Research Institute, UT Southwestern Medical Center, Dallas, TX 75390 USA.
Background:
Glioblastoma (GBM) are highly heterogeneous on the cellular and molecular basis. It has been proposed that glutamine metabolism of primary cells established from human tumors discriminates aggressive mesenchymal GBM subtype to other subtypes.
Methods:
To study glutamine metabolism in vivo, we used a human orthotopic mouse model for GBM. Tumors evolving from the implanted primary GBM cells expressing different molecular signatures were analyzed using mass spectrometry for their metabolite pools and enrichment in carbon 13 (13C) after 13C-glutamine infusion.
Results:
Our results showed that mesenchymal GBM tumors displayed increased glutamine uptake and utilization compared to both control brain tissue and other GBM subtypes. Furthermore, both glutamine synthetase and transglutaminase-2 were expressed accordingly to GBM metabolic phenotypes.
Conclusion:
Thus, our results outline the specific enhanced glutamine flux in vivo of the aggressive mesenchymal GBM subtype.
