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A versatile strategy to synthesize N-methyl-anthranilic acid-labelled glycoprobes for fluorescence-based screening

Isabelle Bertin-Jung1, Anne Robert, Nick Ramalanjaona

  • 1Laboratoire IMoPA UMR 7365 CNRS-Université de Lorraine, Biopôle de la Faculté de Médecine CS 50184, 54505 Vandœuvre-lès-Nancy Cedex, France. sylvie.fournel-gigleux@univ-lorraine.fr.

Chemical Communications (Cambridge, England)
|August 14, 2020
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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