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Solid-phase Synthesis of [4.4] Spirocyclic Oximes
Published on: February 6, 2019
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Multiple Factors Regulate the Spirocyclization Equilibrium of Si-Rhodamines
Fei Deng1,2, Qinglong Qiao1, Jin Li1
1CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
The Journal of Physical Chemistry. B
|August 14, 2020
Summary
Si-rhodamine
Area of Science:
- Chemical Physics
- Biophysical Chemistry
- Materials Science
Background:
- Si-rhodamine is a key fluorophore in super-resolution microscopy.
- Its fluorescence relies on an equilibrium between nonfluorescent lactone and fluorescent zwitterion forms.
- This equilibrium influences its imaging properties.
Purpose of the Study:
- To investigate the environmental factors affecting Si-rhodamine's lactone-zwitterion equilibrium.
- To explore the implications of this equilibrium for its applications.
- To provide guidance for using Si-rhodamine in super-resolution imaging.
Main Methods:
- Studied the effects of molecular aggregation, solvent polarity, pH, metal ions, irradiation, and temperature on Si-rhodamine.
- Evaluated its potential as a hydrochromic material, sensor array, and photoactivatable switch.
- Analyzed its suitability for super-resolution microscopy.
Main Results:
- Si-rhodamine's equilibrium is sensitive to molecular aggregation, solvent polarity, pH, metal ions, irradiation, and temperature.
- These sensitivities enable applications in sensing and switching.
- Environmental factors can induce false signals in super-resolution imaging.
Conclusions:
- Si-rhodamine's environmental sensitivity is a double-edged sword: enabling new applications while requiring careful use in microscopy.
- Understanding these sensitivities is crucial for accurate super-resolution data.
- Further research into controlling this equilibrium could enhance Si-rhodamine's utility.
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