Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

9.3K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.3K
Synthetic Biology02:55

Synthetic Biology

5.3K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.3K
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

16.5K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
16.5K
Genetic Screens02:46

Genetic Screens

5.4K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.4K
Complementary DNA01:44

Complementary DNA

31.0K
Overview
31.0K
Carrier Generation and Recombination01:22

Carrier Generation and Recombination

1.1K
Carrier generation is the process by which electron-hole pairs (EHPs) are created within the semiconductor. In direct-bandgap semiconductors, such as gallium arsenide (GaAs), this occurs efficiently when energy absorption prompts valence electrons to leap into the conduction band, leaving behind holes.
This process is given by the generation rate G and is efficient due to the conservation of momentum between the valence band maximum and conduction band minimum.
Indirect generation involves an...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CD44 restricts EGFR mobility to polarize cytoskeletal signalling modules driving bleb-based migration.

Nature cell biology·2026
Same author

The regulatory logic of a dose-dependent developmental fate decision.

bioRxiv : the preprint server for biology·2026
Same author

Mitoception via the Metabokine GDF15 and Human Health.

Biopsychosocial science and medicine·2026
Same author

Multimodal control of Cas13d activity through domain insertion at an allosteric hotspot.

Nature communications·2026
Same author

Mapping the GDF15 arm of the integrated stress response in human cells and tissues.

Communications biology·2026
Same author

Optogenetic Control of the Integrated Stress Response Limits Glioblastoma Invasion.

Cell biochemistry and function·2026

Related Experiment Video

Updated: Dec 12, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.1K

Engineering combinatorial and dynamic decoders using synthetic immediate-early genes.

Pavithran T Ravindran1,2, Maxwell Z Wilson2,3, Siddhartha G Jena2

  • 1Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ, 08544, USA.

Communications Biology
|August 15, 2020
PubMed
Summary

Researchers engineered synthetic immediate-early genes (SynIEGs) to decode intracellular signaling pathways. This innovation allows for precise control and monitoring of gene expression in response to specific cellular signals.

More Related Videos

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.6K
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.7K

Related Experiment Videos

Last Updated: Dec 12, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.1K
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.6K
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.7K

Area of Science:

  • Molecular Biology
  • Cellular Signaling
  • Synthetic Biology

Background:

  • Cellular functions rely on intracellular signaling pathways controlling gene expression.
  • The precise mechanisms linking signaling pathways to target gene activation are not fully understood.
  • Understanding this logic is key to engineering novel signal-decoding circuits.

Purpose of the Study:

  • To construct synthetic immediate-early genes (SynIEGs) for user-defined regulation of Erk signaling.
  • To develop live-cell biosensors for monitoring SynIEG transcription and translation.
  • To explore SynIEGs for understanding natural gene regulation and engineering synthetic circuits.

Main Methods:

  • Design and construction of synthetic immediate-early genes (SynIEGs).
  • Utilizing live-cell biosensors to track SynIEG transcription and translation.
  • Experimental validation of SynIEG function in response to specific stimuli.

Main Results:

  • Confirmed Erk duration-sensing by the FOS gene using SynIEGs.
  • Elucidated differential regulation of the BTG2 gene by external stimuli.
  • Engineered a SynIEG responsive to combined growth factor and DNA damage signals.

Conclusions:

  • SynIEGs provide a powerful tool for dissecting pathway-decoding logic.
  • This approach enables the engineering of sophisticated molecular circuits for signal decoding.
  • SynIEGs have broad applications in understanding cellular signaling dynamics and engineering synthetic biology systems.