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Related Experiment Videos

Sigma elements are position-specific for many different yeast tRNA genes.

S B Sandmeyer1, V W Bilanchone, D J Clark

  • 1Department of Microbiology and Molecular Genetics, California College of Medicine, University of California, Irvine 92717.

Nucleic Acids Research
|February 25, 1988
PubMed
Summary

Researchers studied the yeast repetitive element, sigma, and its association with transfer RNA (tRNA) genes. Sigma elements are frequently located near tRNA gene transcription start sites, suggesting a functional relationship.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Yeast Research

Background:

  • Repetitive elements in genomes can influence gene regulation and structure.
  • The sigma element is a repetitive DNA sequence found in yeast.
  • Transfer RNA (tRNA) genes are essential for protein synthesis.

Purpose of the Study:

  • To investigate the association between the yeast sigma repetitive element and tRNA genes.
  • To determine the genomic distribution and structural characteristics of sigma elements and related sequences.

Main Methods:

  • DNA sequencing of seventeen sigma elements and flanking regions.
  • Analysis of sequence conservation near tRNA genes.
  • Hybridization analysis of yeast chromosomal DNA using orthogonal field alternation gel electrophoresis (OFAGE).

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Main Results:

  • Fifteen of seventeen analyzed sigma elements initiate near the transcription start site of tRNA genes (-19 to -16 positions).
  • A conserved sequence motif was identified in the region upstream of tRNA genes.
  • Two identical sigma elements form long terminal repeats of a retrotransposon-like element, named Ty3.
  • Ty3 and isolated sigma elements are distributed across multiple yeast chromosomes.

Conclusions:

  • The sigma repetitive element shows a specific association with tRNA genes in the yeast genome.
  • The identified Ty3 element, containing sigma elements, exhibits characteristics of a retrotransposon.
  • Sigma elements and Ty3 are widely distributed throughout the yeast genome, indicating their prevalence and potential impact on genome organization.