Genotoxic and antigenotoxic potential of amygdalin on isolated human lymphocytes by the comet assay

Amygdalin is a cyanogenic glycoside, mainly present in the seeds of the Rosaceae family such as apricots, peaches, and bitter almond. In this study, in vitro genotoxic and antigenotoxic effects of amygdalin have been investigated on human peripheral blood lymphocytes using the comet assay. The antigenotoxic effect of amygdalin was performed against hydrogen peroxide (H₂O₂) using three different treatment types (pre-, simultaneous, and post-treatment). The isolated lymphocytes were incubated with different concentrations of amygdalin (0.86-13.75 µg/ml) alone and in combination with H₂O₂ (100 µM). The results indicated that amygdalin exhibited an antigenotoxic effect against H₂O₂, but it did not induce the genotoxic effect alone in tested concentrations in vitro on human lymphocytes. PRACTICAL APPLICATIONS: Amygdalin is a natural compound used in alternative medicine as an anti-cancer, antipyretic, and cough suppressant. The comet assay which is relatively simple, rapid, sensitive, and economically efficient, measures the changes in genomic stability. Assessment of amygdalin alone has no genotoxic effect on human lymphocytes. Moreover, antigenotoxicity applications (pre-, simultaneous, and post-treatments) of amygdalin significantly reduced the DNA damage induced by H₂O₂ on isolated human lymphocytes. In conclusion, amygdalin is not genotoxic, also, it exhibited antigenotoxic activity against oxidatively damaged DNA due to its antioxidant properties on human lymphocytes.