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Related Concept Videos

Sample Handling01:02

Sample Handling

557
Transportation of samples from the collection point to the laboratory, as well as storage and preservation techniques, are crucial for maintaining sample integrity and ensuring accurate and reliable test results.
Samples should be transported carefully from collection points to the laboratory. They should be properly sealed and clearly labeled to prevent cross-contamination. To preserve the sample integrity, optimal temperature conditions during transport are essential. This could involve using...
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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Updated: Dec 12, 2025

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Cryopreservation without dry ice-induced acidification during sample transport.

Jian Wern Ong1, Tristan Minifie1, Eric Shen Lin1

  • 1Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, 3800, Australia.

Analytical Biochemistry
|August 16, 2020
PubMed
Summary

This study introduces a novel dry ice carrier design that prevents carbon dioxide (CO2) gas from reaching sensitive biological samples during cryogenic transport. The carrier ensures sample integrity and maintains temperatures below -60°C for extended periods.

Keywords:
Carbon dioxideCryogenicDry iceTemperatureTransport

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Area of Science:

  • Biotechnology
  • Cryobiology
  • Biomedical Engineering

Background:

  • Dry ice (solid CO2) is crucial for cryogenic transport of temperature-sensitive biological samples.
  • Sublimated CO2 can compromise sample integrity through acidification.
  • Cryopreservation quality degrades as dry ice depletes.

Purpose of the Study:

  • To develop an improved dry ice carrier for cryogenic transport.
  • To prevent CO2 gas diffusion to biological samples.
  • To ensure sample integrity and safe storage conditions.

Main Methods:

  • Design and testing of a novel dry ice carrier.
  • Evaluation of CO2 gas diffusion prevention.
  • Temperature monitoring below -60°C for 19 hours.
  • Implementation of microcontroller-based temperature and CO2 gas monitoring.

Main Results:

  • The carrier effectively prevents sublimated CO2 from reaching samples.
  • Storage temperature below -60°C was maintained for 19 hours.
  • Integrated monitoring systems provide traceability and safety data.

Conclusions:

  • The described dry ice carrier design enhances the safety and integrity of cryogenic sample transport.
  • This innovation addresses critical challenges in preserving biological samples during transit.
  • The system offers reliable temperature control and gas monitoring for critical biological materials.