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Gold nanoparticle based immunochromatographic biosensor for rapid diagnosis of Mycobacterium avium subspecies
Aditya Agrawal1, Rajat Varshney2, Anil Gattani3
1Division of Animal Biochemistry, IVRI, Izatnagar, Bareilly, U.P. 243122, India.
Abstract:
Highly infectious and obvious withstand ability of Mycobacterium avium subspecies paratuberculosis (MAP) to environment as well as lack of on-site field diagnostic methods notably hampers the paratuberculosis (PTB) control. The existing intricacy, time-consuming and complicated diagnostic methods of PTB accentuate the development of novel and easy-to-perform on-site test. A gold nanoparticle (GNP) based lateral-flow assay (LFA) using MAP recombinant protein (44 kDa) has been developed for sensitive and specific detection of PTB in field conditions. The diagnostic sensitivity and specificity of the LFA for MAP specific antibodies was found approximately 84.2% and 83.3% in comparison to indirect enzyme-linked immunosorbent assay. Consequently, the newly developed GNP based LFA offers on-site and cost-effective method for the prompt diagnosis of PTB and precludes the time-consuming laboratory screening.
Insights
A new gold nanoparticle-based lateral-flow assay (LFA) enables rapid, on-site detection of Mycobacterium avium subspecies paratuberculosis (MAP) antibodies. This cost-effective test aids in controlling paratuberculosis (PTB) by overcoming limitations of current diagnostic methods.
Area of Science:
- Veterinary Diagnostics
- Immunological Assays
- Microbiology
Background:
- Mycobacterium avium subspecies paratuberculosis (MAP) is highly infectious and environmentally persistent.
- Control of paratuberculosis (PTB) is hindered by the lack of rapid, on-site diagnostic tools.
- Current PTB diagnostic methods are complex, time-consuming, and laboratory-dependent.
Purpose of the Study:
- To develop a novel, easy-to-perform, on-site diagnostic test for PTB.
- To create a sensitive and specific assay for detecting MAP infection in field conditions.
- To provide a cost-effective alternative to traditional laboratory screening for PTB.
Main Methods:
- Development of a gold nanoparticle (GNP) based lateral-flow assay (LFA).
- Utilized a MAP recombinant protein (44 kDa) as the detection antigen.
- Compared LFA performance against indirect enzyme-linked immunosorbent assay (ELISA).
Main Results:
- The GNP-based LFA demonstrated diagnostic sensitivity of approximately 84.2%.
- The LFA achieved a diagnostic specificity of approximately 83.3%.
- The assay is suitable for sensitive and specific detection of MAP-specific antibodies.
Conclusions:
- The developed GNP-based LFA provides a rapid, on-site diagnostic method for PTB.
- This assay is a cost-effective tool for prompt PTB diagnosis in field settings.
- The LFA circumvents the need for time-consuming laboratory screening, aiding PTB control efforts.
