Biohydrogen production beyond the Thauer limit by precision design of artificial microbial consortia

Dark fermentative biohydrogen (H₂) production could become a key technology for providing renewable energy. Until now, the H₂ yield is restricted to 4 moles of H₂ per mole of glucose, referred to as the "Thauer limit". Here we show, that precision design of artificial microbial consortia increased the H₂ yield to 5.6 mol mol^-1 glucose, 40% higher than the Thauer limit. In addition, the volumetric H₂ production rates of our defined artificial consortia are superior compared to any mono-, co- or multi-culture system reported to date. We hope this study to be a major leap forward in the engineering of artificial microbial consortia through precision design and provide a breakthrough in energy science, biotechnology and ecology. Constructing artificial consortia with this drawing-board approach could in future increase volumetric production rates and yields of other bioprocesses. Our artificial consortia engineering blueprint might pave the way for the development of a H₂ production bioindustry.