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Related Experiment Video

Updated: Dec 11, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
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MicroRNA-15b Targets VEGF and Inhibits Angiogenesis in Proliferative Diabetic Retinopathy.

Ying Yang1, Yan Liu2, Yiping Li1

  • 1Department of Endocrinology, The Second People's Hospital of Yunnan Province, Kunming, Yunnan, China.

The Journal of Clinical Endocrinology and Metabolism
|August 16, 2020
PubMed
Summary

Low levels of microRNA-15b (miR-15b) are linked to high vascular endothelial growth factor (VEGF) in proliferative diabetic retinopathy (PDR). Targeting miR-15b may help regulate VEGF and reduce angiogenesis in PDR.

Keywords:
angiogenesisdiabetesdiabetic retinopathymicroRNA-15bproliferative diabetic retinopathyvascular endothelial growth factor

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Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Endocrinology

Background:

  • Diabetic retinopathy (DR) involves retinal microvascular changes.
  • Vascular endothelial growth factor (VEGF) drives angiogenesis in proliferative diabetic retinopathy (PDR).
  • MicroRNAs (miRs) are implicated in VEGF-mediated pathological processes.

Purpose of the Study:

  • Investigate the role of specific miRs associated with VEGF in PDR.
  • Determine the relationship between circulating miR-15b and VEGF levels in diabetic patients.

Main Methods:

  • RNA sequencing identified differentially expressed miRs in diabetes mellitus (DM), nonproliferative diabetic retinopathy (NPDR), and PDR.
  • Quantitative real-time PCR measured serum miR-15b concentrations.
  • In vivo studies in diabetic rats assessed miR-15b effects on vascularization and VEGF.

Main Results:

  • Circulating miR-15b levels were inversely correlated with VEGF levels in PDR patients.
  • miR-15b directly targets VEGF's 3'-untranslated region, inhibiting its transcription.
  • Overexpression of miR-15b suppressed in vivo angiogenesis and VEGF expression in diabetic rats.

Conclusions:

  • Circulating miR-15b is a potential biomarker associated with PDR.
  • miR-15b can regulate VEGF expression and subsequent angiogenesis.
  • Targeting miR-15b may offer a therapeutic strategy for PDR.