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Frozen Blood Reserves

Johan W Lagerberg1

  • 1Department of Blood Cell Research, Sanquin Research, Amsterdam, The Netherlands. j.lagerberg@sanquin.nl.

Methods in Molecular Biology (Clifton, N.J.)
|August 16, 2020
PubMed

Frozen blood reserves are an important component in meeting blood needs. The idea behind a frozen blood reserve is twofold: to freeze units of rare blood types for later use by patients with special transfusion needs and for managing special transfusion circumstances. The permeating additive glycerol is used as a cryoprotectant to protect red blood cells (RBCs) from freezing damage. The use of thawed RBCs has been hampered by a 24-h outdating period due to the potential bacterial contamination when a functionally open system is used for addition and removal of the glycerol. The introduction of an automated, functionally closed system for glycerolization and deglycerolization of RBCs improved the operational practice. More importantly, the closed process allowed for extended shelf life of the thawed RBCs. In the current chapter, a cryopreservation procedure for RBCs using a functionally closed processing system is described.

Keywords:
CryopreservationGlycerolRed blood cellsSAGM

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