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Related Experiment Video

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In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
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Stallion Semen Cooling Using Native Phosphocaseinate-based Extender and Sodium Caseinate Cholesterol-loaded

Guilherme Novello1, Giorgia Podico1, Lorenzo G T M Segabinazzi1

  • 1Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, Urbana, IL.

Journal of Equine Veterinary Science
|August 17, 2020
PubMed
Summary

BotuSemen Gold demonstrated superior motility and sperm velocity compared to INRA 96 for cooled stallion semen. Both extenders yielded identical embryo recovery rates, suggesting BotuSemen Gold as a viable alternative for clinical use.

Keywords:
HorsesPassive semen cooling deviceSemen extender

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Area of Science:

  • Veterinary reproduction
  • Semen cryopreservation
  • Equine science

Background:

  • Optimizing stallion semen extenders is crucial for maintaining sperm viability during cooling.
  • INRA 96 is a widely used extender, but alternatives are sought to improve post-thaw semen quality.

Purpose of the Study:

  • To compare the efficacy of BotuSemen Gold and INRA 96 extenders for cooled stallion semen.
  • To evaluate the impact of extenders on sperm parameters and in vivo embryo recovery rates.

Main Methods:

  • Stallion ejaculates were extended with INRA 96 or BotuSemen Gold and cooled for 48 hours.
  • Sperm motility, plasma membrane integrity, and mitochondrial membrane potential were assessed.
  • Fertility was evaluated via embryo recovery rates after artificial insemination.

Main Results:

  • BotuSemen Gold showed significantly higher total and progressive motility than INRA 96 (P < .05).
  • BotuSemen Gold exhibited superior sperm velocity parameters throughout the cooling period.
  • No significant differences were observed in motility between extenders after cushion-centrifugation, but BotuSemen Gold maintained better velocity.
  • Embryo recovery rates were identical (50%) for both extenders.

Conclusions:

  • BotuSemen Gold is a suitable alternative to INRA 96 for cooled stallion semen preservation.
  • The choice of extender can influence sperm motility and velocity, impacting field fertility outcomes.