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Updated: Dec 11, 2025

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Nitrogen-doping to enhance the separation selectivity of glucose-based carbon dots-modified silica stationary phase
Ning Yuan1, Jia Chen2, Hang Zhou2
1CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China; Laboratory on Pollution Monitoring and Control, College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, 830054, China.
Abstract:
Doping is often used to alter or improve the properties of nanomaterials. In this work, a novel glucose-based nitrogen-doped carbon dots (Glc-NCDs)-bonded silica stationary phase (Sil-Glc-NCDs) was synthesized and characterized carefully. Glc-NCDs were prepared using glucose and aspartic acid as carbon and nitrogen sources, and then grafted on silica surface via isocyanatopropyl as linker using deep eutectic solvents as reaction medium. The synthesis was confirmed by elemental analysis, Fourier transform infrared spectroscopy and microscopic imaging techniques. And then Sil-Glc-NCDs were packed in the stainless columns for hydrophilic interaction chromatography (HILIC). Due to the synergistic effect between the functional groups of doped carbon dots, Sil-Glc-NCDs column showed enhanced separation selectivity compared with previous non-doped Sil-Glc-CDs column. Sil-Glc-NCDs column can be used for separation of base, nucleosides and antibiotics etc. This column was successfully applied to determine the content of roxithromycin in the capsule, which was found that the concentration was 2.45 mg/mL.
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