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Updated: Dec 11, 2025
A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Development of luminol-fluorescamine-PVP chemiluminescence system and its application to sensitive tyrosinase
Chao Wang1, Yequan Chen1, Dmytro Snizhko2
1College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China.
Abstract:
Fluorescamine is a popular fluorescent probe. We report for the first time that luminol chemiluminescence (CL) can be enhanced by fluorescamine in the presence of PVP. The CL intensity of luminol-fluorescamine-PVP is about 26 times stronger than that of luminol. Both the removal of oxygen and the addition of superoxide dismutase (SOD) decrease CL intensity, thiourea and NaN3 have little effect on CL intensities, indicating that O2•- is critical for CL. Interestingly, o-quinone generated from phenol by tyrosinase obviously inhibited the CL intensity. Inspired by such quenching effect on the luminol-fluorescamine-PVP CL system, a sensitive CL sensing for the determination of tyrosinase activity was developed. The method can detect tyrosinase in the range of 0.07-1.5 μg mL-1 (0.19-4.02 U mL-1) with the detection limit of 0.035 μg mL-1 (0.094 U mL-1). Moreover, this method exhibits satisfied recoveries for the spiked human serum samples.
