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Experimental sulphide inhibition calibration method in nitrification processes: A case-study.

G Noriega-Hevia1, O Mateo1, A Maciá2

  • 1CALAGUA, Unidad Mixta UV-UPV, Institut Universitari D'Investigació D'Enginyeria de L'Aigua I Medi Ambient, IIAMA, Universitat Politècnica de València, Camí de Vera S/n, 46022, València, Spain.

Journal of Environmental Management
|August 18, 2020
PubMed
Summary

Sulphide inhibits nitrification in wastewater treatment plants (WWTPs). This study developed a method to measure sulphide's effect on ammonia-oxidising bacteria (AOB) and nitrite-oxidising bacteria (NOB), finding NOB more sensitive.

Keywords:
Ammonia-oxidising bacteria calibrationCalibration methodologyNitrification inhibitionNitrite-oxidising bacteria calibrationSulphide inhibition

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Area of Science:

  • Environmental Science
  • Microbiology
  • Water Treatment Engineering

Background:

  • Sulphide is a known nitrification inhibitor in wastewater treatment plants (WWTPs), particularly in regions with sulphate-rich soils.
  • Limited information exists regarding the specific mechanisms and extent of sulphide's inhibitory effects on key microbial players in nitrification.

Purpose of the Study:

  • To develop and apply a modified Successive Additions Method for calibrating sulphide's inhibitory effect on ammonia-oxidising bacteria (AOB) and nitrite-oxidising bacteria (NOB).
  • To investigate the differential sensitivity of AOB and NOB to sulphide exposure in activated sludge from WWTPs with varying influent sulphide levels.

Main Methods:

  • Modification of the Successive Additions Method to assess sulphide inhibition.
  • Application of the developed method to activated sludge samples from two WWTPs with different influent sulphide concentrations.
  • Modeling of AOB and NOB activity using the Hill inhibition equation.

Main Results:

  • Sulphide exhibited a greater inhibitory effect on nitrite-oxidising bacteria (NOB) compared to ammonia-oxidising bacteria (AOB).
  • Activated sludge from WWTPs with higher influent sulphide concentrations showed lower sulphide inhibition.
  • The Hill inhibition equation accurately modeled the observed AOB and NOB activity across different sulphide concentrations.

Conclusions:

  • The developed method provides a reliable way to quantify sulphide inhibition on nitrification bacteria.
  • Nitrification bacteria in sulphide-rich environments may exhibit increased tolerance to sulphide.
  • Understanding these inhibition dynamics is crucial for optimizing WWTP performance in sulphide-affected areas.