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Updated: Dec 11, 2025
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Frequency and comparison among antibiotic resistant Staphylococcus aureus strains in selected hospitals of Peshawar,
Sajjad Ahmad1, Shehzad Ahmed1, Muhammad Shehryar Sabir1
1Department of Microbiology, Hazara University, Mansehra, KPK, Pakistan.
Objective:
To assess and compare the frequency of antibiotic-resistant staphylococcus aureus strains.
Methods:
A retrospective study was conducted at the privately-owned Welfare Medical Laboratory, Peshawar, Pakistan, and comprised record related to the period between 07th February 2017 and 23rd March 2018 of patients referred for pus-testing from Khyber Teaching Hospital, Lady Reading Hospital and the Hayatabad Medical Complex. Pus samples were obtained from various parts of body with cotton swabs. The samples were cultured and the isolated staphylococcus aureus strains were analysed against selected antibiotics. The frequency of the isolated strains was tested and compared using Prism 7 software.
Results:
Of the 6780 samples, staphylococcus aureus was found in 4315(63.64%). Wild-type staphylococcus aureus strains were 2133(31.46%), followed by 825(12.16%) methicillin resistant, 792(11.68%) vancomycin intermediate, and 565(8.33%) vancomycin-resistant staphylococcus aureus strains. The isolated strains were significant (p<0.0001) for operated wounds, and non-significant (p=0.8915) for diabetic foot cases.
Conclusions:
The frequency of antibiotic-resistant staphylococcu saureus strains was high.
Insights
Antibiotic-resistant Staphylococcus aureus strains were frequently identified in pus samples. This study highlights a high prevalence of resistant strains, including methicillin-resistant and vancomycin-resistant Staphylococcus aureus.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Clinical Pathology
Background:
- Staphylococcus aureus is a significant human pathogen.
- Antibiotic resistance in Staphylococcus aureus is a growing global health concern.
- Understanding resistance patterns is crucial for effective treatment.
Purpose of the Study:
- To determine the frequency of antibiotic-resistant Staphylococcus aureus strains.
- To compare resistance patterns among different Staphylococcus aureus strains.
- To analyze resistance in relation to patient wound types.
Main Methods:
- Retrospective analysis of pus samples cultured between February 2017 and March 2018.
- Identification and susceptibility testing of isolated Staphylococcus aureus strains against various antibiotics.
- Statistical comparison of strain frequencies using Prism 7 software.
Main Results:
- Staphylococcus aureus was detected in 63.64% of 6780 samples.
- Methicillin-resistant Staphylococcus aureus (MRSA) accounted for 12.16% of isolates.
- Vancomycin-intermediate and vancomycin-resistant strains were also prevalent (11.68% and 8.33%, respectively).
Conclusions:
- A high frequency of antibiotic-resistant Staphylococcus aureus strains was observed.
- Resistance patterns showed significant association with operated wounds.
- The findings underscore the need for vigilant antibiotic stewardship.
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