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Updated: Dec 11, 2025

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
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Distinct transcriptional programs of SOX2 in different types of small cell lung cancers

Yuki Tenjin1,2, Kumi Matsuura3, Shinji Kudoh1

  • 1Department of Pathology and Experimental Medicine, Graduate School of Medical Science, Kumamoto University, Honjo 1-1-1, Chuo-ku, Kumamoto, 860-8556, Japan.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|August 18, 2020
PubMed

Insights

SOX2 is an oncogene in small cell lung cancer (SCLC). Targeting the ASCL1-SOX2 axis shows therapeutic potential in the SCLC-A subtype, but not SCLC-Y.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • SOX2 is an oncogene in small cell lung cancer (SCLC), an aggressive neuroendocrine tumor.
  • The precise role of SOX2 and targeted therapeutic strategies in SCLC remain incompletely understood.

Purpose of the Study:

  • To elucidate the role of SOX2 in different SCLC subtypes.
  • To investigate the ASCL1-SOX2 signaling axis and its therapeutic implications.

Main Methods:

  • Next-generation sequencing to analyze SOX2 dependency on ASCL1 in SCLC-A.
  • Immunohistochemistry to assess SOX2, ASCL1, and INSM1 expression in SCLC tissues.
  • SOX2 knockdown experiments in SCLC-A and SCLC-Y subtypes.

Main Results:

  • SOX2 expression in SCLC-A is ASCL1-dependent, promoting NE differentiation and tumorigenesis via INSM1 and WNT11.
  • SOX2 targets Hippo pathway genes in the ASCL1-negative SCLC-Y subtype.
  • SOX2 knockdown inhibited SCLC-A proliferation but had limited effect on SCLC-Y growth.

Conclusions:

  • The ASCL1-SOX2 axis is crucial for the SCLC-A subtype.
  • SOX2 plays distinct roles in SCLC-A and SCLC-Y subtypes.
  • Targeting the ASCL1-SOX2 axis presents a potential therapeutic strategy for SCLC-A.

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