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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
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Updated: Dec 11, 2025

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Long Noncoding RNA ADAMTS9-AS2 Inhibits the Proliferation, Migration, and Invasion in Bladder Tumor Cells.

Zhan Zhang1, Jin-Peng Jia2, Yin-Jiang Zhang3

  • 1Department of Rehabilitation Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, People's Republic of China.

Oncotargets and Therapy
|August 18, 2020
PubMed
Summary

The long noncoding RNA ADAMTS9-AS2 acts as a tumor suppressor in bladder cancer. It inhibits cell proliferation, migration, and invasion, potentially via the PI3K/AKT/mTOR pathway.

Keywords:
PI3K/AKT/mTORbladder tumorlncRNA ADAMTS9-AS2proliferation

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An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment
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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Bladder cancer is a prevalent malignancy with incompletely understood pathogenesis.
  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cancer, but many remain uncharacterized.
  • The lncRNA ADAMTS9-AS2 has been implicated in various cancers, but its function in bladder cancer was unexplored.

Purpose of the Study:

  • To investigate the role and potential mechanism of lncRNA ADAMTS9-AS2 in bladder tumor cells.
  • To determine if ADAMTS9-AS2 expression correlates with bladder tumor progression.
  • To explore ADAMTS9-AS2's impact on cell proliferation, migration, and invasion.

Main Methods:

  • Quantitative real-time PCR and bioinformatic analyses to assess ADAMTS9-AS2 and ADAMTS9 expression.
  • Western blot assays to evaluate protein levels of key molecules.
  • Cell proliferation (CCK8, clonogenic assays), migration (scratch wound healing), and invasion (Transwell assays) were performed.

Main Results:

  • ADAMTS9-AS2 was found to be downregulated in bladder tumor cells.
  • Overexpression of ADAMTS9-AS2 suppressed proliferation, migration, and invasion.
  • ADAMTS9-AS2 expression positively correlated with ADAMTS9 and was linked to the PI3K/AKT/mTOR pathway, autophagy, and apoptosis-related proteins.

Conclusions:

  • ADAMTS9-AS2 functions as a tumor suppressor in bladder cancer, inhibiting cell proliferation, migration, and invasion.
  • The mechanism involves regulation of the PI3K/AKT/mTOR pathway and key autophagy/apoptotic proteins.
  • ADAMTS9-AS2 shows potential as a diagnostic biomarker for bladder cancer patients.