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Long Noncoding RNA ST7-AS1 Upregulates TRPM7 Expression by Sponging microRNA-543 to Promote Cervical Cancer

Hongguo Qi1, Lianwei Lu2, Lili Wang3

  • 1Department of Gynaecology and Obstetrics, The Second Children & Women's Healthcare of Jinan City, Jinan, Shandong 250022, People's Republic of China.

Oncotargets and Therapy
|August 18, 2020
PubMed
Abstract

Insights

The long noncoding RNA ST7 antisense RNA 1 (ST7-AS1) promotes cervical cancer progression by upregulating TRPM7 via sponging miR-543. Targeting this ST7-AS1/miR-543/TRPM7 axis offers a potential therapeutic strategy for cervical cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) play critical roles in various cancers.
  • ST7 antisense RNA 1 (ST7-AS1) has been implicated in gastric and laryngeal cancers.
  • The role of ST7-AS1 in cervical cancer remains largely unexplored.

Purpose of the Study:

  • To investigate the expression and function of ST7-AS1 in cervical cancer.
  • To elucidate the underlying molecular mechanisms of ST7-AS1 in promoting cervical cancer progression.
  • To evaluate the therapeutic potential of targeting the ST7-AS1 pathway.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) for ST7-AS1 expression analysis.
  • Cell proliferation, apoptosis, migration, and invasion assays to assess cellular malignancy.
  • Bioinformatics, luciferase reporter, and RNA immunoprecipitation assays to identify molecular interactions.
  • Xenograft experiments for in vivo tumor growth evaluation.

Main Results:

  • ST7-AS1 was significantly upregulated in cervical cancer tissues and cell lines.
  • ST7-AS1 overexpression correlated with advanced disease stage, lymph node metastasis, and poor survival.
  • ST7-AS1 promoted proliferation, migration, and invasion while inhibiting apoptosis; it also enhanced tumor growth in vivo.
  • ST7-AS1 acted as a competing endogenous RNA (ceRNA) by sponging miR-543 to upregulate transient receptor potential melastatin 7 (TRPM7) expression.

Conclusions:

  • The ST7-AS1/miR-543/TRPM7 axis is a novel pathway driving cervical cancer oncogenicity.
  • ST7-AS1 inhibition effectively suppressed cervical cancer progression both in vitro and in vivo.
  • This axis represents a promising therapeutic target for cervical cancer treatment.
Keywords:
ST7 antisense RNA 1cervical cancermicroRNA-543transient receptor potential melastatin 7

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