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Updated: Dec 11, 2025

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TBRG4 Knockdown Suppresses Proliferation and Growth of Human Osteosarcoma Cell Lines MG63 Through PI3K/Akt Pathway

Fei Huang1, Faxue Liao1, Guangwen Ma1

  • 1Department of Orthopaedics, The Fourth Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People's Republic of China.

Oncotargets and Therapy
|August 18, 2020
PubMed
Abstract

Insights

Transforming growth factor β regulator 4 (TBRG4) is upregulated in osteosarcoma (OS). Inhibiting TBRG4 suppresses OS cell growth and promotes apoptosis, suggesting TBRG4 as a potential therapeutic target for OS.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Transforming growth factor β regulator 4 (TBRG4) is implicated in various cancers.
  • The role of TBRG4 in human osteosarcoma (OS) remains largely unknown.

Purpose of the Study:

  • To investigate the expression and function of TBRG4 in human osteosarcoma.
  • To explore TBRG4 as a potential therapeutic target for osteosarcoma.

Main Methods:

  • Immunohistochemistry and quantitative real-time PCR to assess TBRG4 expression in OS tissues and cell lines.
  • TBRG4 knockdown using lentivirus to evaluate its effects on cell proliferation, colony formation, and apoptosis.
  • Bioinformatics analysis and Western blot to identify associated signaling pathways.

Main Results:

  • TBRG4 expression was significantly elevated in OS tissues and the MG63 cell line.
  • TBRG4 knockdown inhibited cell proliferation, colony formation, and tumor growth.
  • TBRG4 knockdown promoted apoptosis and was found to regulate the PI3K/Akt signaling pathway.

Conclusions:

  • TBRG4 plays a crucial role in osteosarcoma progression.
  • TBRG4 represents a promising therapeutic target for human osteosarcoma treatment.
Keywords:
MG63 cellsPI3K/AktTBRG4osteosarcomaproliferation

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