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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
Published on: January 7, 2019
M-protein based vaccine induces immunogenicity and protection from Streptococcus pyogenes when delivered on a
Jamie-Lee S Mills1, Cesar M Jayashi Flores2, Simone Reynolds1
1Institute for Glycomics, Griffith University, Gold Coast, Australia.
Vaccination using the J8-DT/HD-MAP, a novel microarray patch delivery system, shows strong antibody responses and significant protection against Streptococcus pyogenes skin infections in mice. This new vaccine technology offers potential advantages over traditional methods.
Area of Science:
- Immunology
- Vaccinology
- Microbiology
Background:
- Streptococcus pyogenes (S. pyogenes) is a significant human pathogen causing various infections.
- Developing effective vaccines against S. pyogenes remains a public health priority.
- Novel vaccine delivery systems are being explored to improve immunogenicity and ease of administration.
Purpose of the Study:
- To evaluate the efficacy of a candidate S. pyogenes vaccine (J8-DT) delivered via a high-density microarray patch (HD-MAP).
- To compare the immunogenicity and protective efficacy of J8-DT/HD-MAP with conventional intramuscular vaccination.
- To assess the impact of dose reduction and vaccination frequency using the HD-MAP system.
Main Methods:
- Mice were vaccinated with J8-DT/HD-MAP or J8-DT adjuvanted with Alum (J8-DT/Alum).
- Antibody responses (total IgG, isotype profile) were measured.
- Vaccine efficacy was assessed through a S. pyogenes skin challenge, quantifying bacterial load in skin and blood.
- Dose-ranging and frequency studies were conducted for the HD-MAP formulation.
Main Results:
- J8-DT/HD-MAP induced comparable total IgG responses to J8-DT/Alum.
- Reduced vaccination frequency (two doses) with J8-DT/HD-MAP yielded similar antibody levels to three doses of J8-DT/Alum.
- J8-DT/HD-MAP vaccination significantly reduced S. pyogenes in skin (92.9%) and blood (100%) compared to unadjuvanted J8-DT, with protection comparable to J8-DT/Alum.
- J8-DT/HD-MAP induced a Th1-biased antibody response, while J8-DT/Alum showed a Th2 bias.
Conclusions:
- J8-DT/HD-MAP is a promising vaccine candidate for S. pyogenes, demonstrating comparable immunogenicity and protection to conventional methods.
- The HD-MAP delivery system offers potential advantages including dose reduction, improved stability, ease of use, and reduced waste.
- Further clinical development of J8-DT/HD-MAP is warranted for S. pyogenes control programs.
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