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Updated: Dec 11, 2025

Extraction and Analysis of Cortisol from Human and Monkey Hair
Published on: January 24, 2014
Hair cortisol and dehydroepiandrosterone concentrations: Associations with executive function in early childhood
Ella-Marie P Hennessey1, Olga Kepinska2, Stephanie L Haft3
1Department of Psychiatry and Weill Institute for Neurosciences and Dyslexia Center, University of California San Francisco, 401 Parnassus Ave., San Francisco, CA, 94143, USA; Multi-University Precision Learning Center, 401 Parnassus Ave., San Francisco, CA, 94143, USA; Department of Psychology, University of Denver, 2155 S. Race Street, Denver, CO, 80210, USA.
Abstract:
Chronic stress during childhood negatively impacts cognition and physical and mental health. Exposure to stressors over time can cause hypothalamic-pituitary-adrenal (HPA) axis dysregulation, leading to abnormal stress hormone levels, which can be reflected in hair cortisol concentration (HCC) and hair dehydroepiandrosterone (DHEA) concentration. Although the use of HCC and DHEA to measure chronic stress in children is increasing, their effects on cognition (as indexed by executive function) remain unexplored. Accordingly, we aimed to investigate the associations of HCC, DHEA, and their ratio with measures of executive function (cognitive flexibility and working memory) in a sample of kindergarten children (N=100). We found that the expected negative association between HCC and working memory approached significance, and DHEA was significantly and positively related to cognitive flexibility. We discuss possible interpretations of our findings. Our results suggest promising areas for future investigation and encourage further exploration into HCC and DHEA as measures of chronic stress.
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