Can DL_NO/DL_CO ratio offset prejudicial effects of functional heterogeneities in acinar regions?

(1) To establish the general equation that describes relationship of DM_CO/Vc versus DL_NO/DL_CO under conditions with no functional heterogeneities. (2) To examine the effects of functional heterogeneities, including parallel and series (stratified) heterogeneities, on DL_NO/DL_CO.

(1) Given that "true" θ_NO in pulmonary capillaries is represented by surface absorption-related θ_NO, relationship between DM_CO/Vc and DL_NO/DL_CO does not differ significantly from that obtained on premise of infinite θ_NO. DL_NO/DL_CO decided physiologically may mirror morphometric DM_CO/Vc actually working for gas exchange but not "total" morphometric ratio of DM_CO/Vc. (2) There are three parallel heterogeneities that affect diffusing capacity (D)-related parameters. Of them, only the heterogeneity of D/V_A, where V_A is alveolar volume, underestimates DL_CO and DL_NO. DL_NO/DL_CO does not alleviate negative impact of D/V_A heterogeneity, indicating that DM_CO/Vc estimated from DL_NO/DL_CO does not mirror "true" morphometric DM_CO/Vc in diseased lungs with D/V_A maldistribution. (3) Stratified heterogeneity underrates morphometric DM_CO, DM_NO, and DM_NO/DM_CO maximally by 1.4 %, 2.8 %, and 1.4 %, respectively, under conditions similar to single-breath D measurements, suggesting that effect of stratified heterogeneity on D measures is no longer needed to be considered in normal subjects but may be in patients having lung diseases with destructive lesions of acinar structures.