Molecular characterization of Staphylococcus aureus complex from fomites in Nigeria

Fomites serve as a potential route for the transmission of pathogens including community-acquired methicillin-resistant Staphylococcus aureus to humans. Phylogenetic and taxonomic analyses have established the Staphylococcus aureus complex (S. aureus, S. argenteus and S. schweitzeri), however, phenotypic characteristics are insufficient in the delineation of these species. In this study, we describe the S. aureus complex from inanimate surfaces in Nigeria. Fomite samples in Obafemi Awolowo University were initially screened for S. aureus and species differentiation was determined by MALDI-TOF, PCR of the S. aureus specific thermonuclease and the nonribosomal peptide synthetase genes. Characterization of the isolates was based on antimicrobial susceptibility, spa typing, multilocus sequence typing and virulence gene detection (lukS/lukF-PV, chp, sak, scn). Whole-genome sequencing was done for selected isolates. Of the 239 fomites samples, 14  S. aureus and two S. schweitzeri isolates were identified including three MRSA. Genotyping classified the S. aureus isolates into ST8/CC8, ST30/CC30, ST15/ST5875/CC15, ST508/ST5876/CC45, ST121/CC121, ST152/CC152 and ST3961. All the isolates in CC30, CC121, and CC152 were lukS/lukF-PV positive. The MRSA (PVL+) were assigned with CC152. Phylogenetic analysis revealed that the S. schweitzeri isolates were closely related with those from fruit bats (Eidolon helvum) in Nigeria. The differentiation of S. aureus from S. schweitzeri was clearly achieved through MALDI-TOF and PCR. Fomites are not only a reservoir for S. aureus but also for S. schweitzeri that was so far recovered primarily in African wildlife.