Anammox process dosed with biochars for enhanced nitrogen removal: Role of surface functional groups

Biochar is an inexpensive redox-active carbon material that has been demonstrated to enhance microbial nitrogen-transforming processes. However, how redox-active biochar affects anammox remains unclear. Here, the effects of three functionally distinct biochars produced from corn stover biomass at varied pyrolysis temperatures (CS300, CS500, CS800) were evaluated as additives on the anammox performance in three reactors (R300, R550, R800) over the long term, during which nitrogen loading rate was either increased drastically (pulse strategy) or gradually (gradual strategy). Nitrogen removal was achieved at 86.5% (R300), 77.1% (Control), 59.3% (R550) and 57.7% (R800) under pulse strategy, and at 95.4% (R300), 92.3% (R550), 86.2% (Control) and 82.0% (R800) under gradual strategy, respectively. Compared with Control, addition of CS300 increased abundance of Candidatus Kuenenia with superior anammox activity. CS300 enriched with reduced functional groups (phenolic/hydroquinone) could donate electrons to support bioenergetics of anammox metabolism, whereas electron-accepting CS800 functioned inversely. Overall, this study highlights the importance of surface functional groups and redox property of biochar such that determines whether its addition impose stimulatory or suppressive effect on anammox process.