Biodegradation of Phenol by Rhodococcus sp. Strain SKC: Characterization and Kinetics Study

This study focuses on the kinetics of a pure strain of bacterium Rhodococcus sp. SKC, isolated from phenol-contaminated soil, for the biodegradation of phenol as its sole carbon and energy source in aqueous medium. The kinetics of phenol biodegradation including the lag phase, the maximum phenol degradation rate, maximum growth rate (R) and maximum yield coefficient (Y) for each S_i (initial phenol concentration, mg/L) were fitted using the Gompertz and Haldane models of substrate inhibition (R² > 0.9904, RMSE < 0.00925). The values of these parameters at optimum conditions were μ_max = 0.30 h^-1, K = 36.40 mg/L, and K_i = 418.79 mg/L, and that means the inhibition concentration of phenol was 418.79 mg/L. By comparing with other strains of bacteria, Rhodococcus sp. SKC exhibited a high yield factor and tolerance towards phenol. This study demonstrates the potential application of Rhodococcus sp. SKC for the bioremediation of phenol contaminate.